Western blot of nonreduced(-) and reduced(+) ELISA of mouse immunoglobulins shows RM106 A titer ELISA of mouse IgG1. The plate was coated
Product Name Biotin Anti-Mouse IgG1 Rabbit Monoclonal Antibody [RM106]
Product Description Biotinylated Rabbit monoclonal to Mouse IgG1
Catalog No. 31-1002-02
Clone Name RM106
Product Data Sheet Biotin Anti-Mouse IgG1 Rabbit Monoclonal Antibody Data Sheet RM106
Specificity RM106 reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
The Fc region of RM106 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgG
Application Western Blot (WB)
Flow Cytometry (FC)
Species Reactivity Mouse
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 ug/mL-0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords . Product Images
mouse IgG1 (20ng/lane), using 0.2ug/mL of
RevMAb clone RM106. This antibody reacts to
nonreduced IgG1 (~150 kDa) stronger than the
reduced γ1 form (~50 kDa).
reacts to the Fc region of mouse IgG1; no cross
reactivity with IgG2a, IgG3, IgM, IgA, IgE, human
IgG, or rat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM106 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.
with different amount of mouse IgG1. A serial dilution
of RM106 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as
the secondary antibody.
Western blot of nonreduced(-) and reduced(+)
ELISA of mouse immunoglobulins shows RM106
A titer ELISA of mouse IgG1. The plate was coated