Biotin Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM103

$280.00

Biotinylated Rabbit monoclonal to Mouse Ig Kappa Light Chain

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SKU: 31-1007-02.

Product Details
Product NameBiotin Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody [RM103]
Product DescriptionBiotinylated Rabbit monoclonal to Mouse Ig Kappa Light Chain
Catalog No.31-1007-02
Clone NameRM103
Product Data SheetBiotin Anti-Mouse Ig Kappa Rabbit Monoclonal Antibody Data Sheet RM103
SpecificityThis antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG.
The Fc region of RM103 has been engineered to eliminate Fc receptor binding.
ImmunogenMouse IgG
ApplicationWestern Blot (WB) (nonreduced)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateBiotin
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50 ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005ug/mL-0.2ug/mL;
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL;
Western Blot (WB): 0.1ug/mL-0.5ug/mL.
Keywords
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Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mL
of RevMAb clone RM103. This antibody reacts to
nonreduced IgG1κ (~150 kDa), and slightly reacts
to reduced κ light chain (~25 kDa).

ELISA

ELISA of mouse immunoglobulins shows RM103
reacts to the kappa light chain of mouse immuno-
globulins. No cross reactivity with the lamda light
chain, human IgG, rat IgG, or goat IgG. The plate
was coated with 50 ng/well of different immuno-
globulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of
RM103 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.

A

A titer ELISA of mouse IgG1κ. The plate was coated
with different amounts of mouse IgG1κ. A serial
dilution of RM103 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.


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