Western blot of nonreduced (-) and reduced (+) ELISA of mouse immunoglobulins shows RM104 A titer ELISA of mouse IgG. The plate was coated
Product Details
Product Name Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone RM104
Product Description Biotinylated Rabbit monoclonal to Mouse IgG
Catalog No. 31-1001-02
Clone Name RM104
Product Data Sheet Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody Data Sheet RM104
Specificity RM104 reacts to the Fc region of all mouse IgG subclasses. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.
The Fc region of RM104 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgG
Application ELISA
Western Blot (WB) (nonreduced)
Species Reactivity Mouse
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 µg/mL - 0.2 µg/mL;
Western Blot (WB): 0.1 µg/mL - 0.5 µg/mL.
Keywords
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Product Images
mouse immunoglobulins (20 ng/lane), using
0.2 µg/mL of RevMAb Clone RM104. This antibody
reacts to nonreduced mouse IgG1, IgG2a, IgG2b,
and IgG3. It showed no cross reactivity with IgM,
IgA, or IgE.
reacts to the Fc region of mouse IgG1, IgG2a,
IgG2b, and IgG3; no cross reactivity with IgM,
IgA, IgE, human or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
with different amounts of mouse IgG. A serial dilution
of RM104 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as
the secondary antibody.
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