Western blot of nonreduced(-) and reduced(+) ELISA of mouse immunoglobulins shows RM107 A titer ELISA of mouse IgG2aκ. The plate was
Product Name Biotin Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody [RM107]
Product Description Biotinylated Rabbit monoclonal to Mouse IgG2a Kappa
Catalog No. 31-1003-02
Clone Name RM107
Product Data Sheet Biotin Anti-Mouse IgG2a Kappa Light Chain Rabbit Monoclonal Antibody Data Sheet RM107
Specificity RM107 reacts to the Fab region of mouse IgG2aκ. No cross reactivity with mouse IgG2aΚ, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM107 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgG
Application Western Blot (WB) (nonreduced only)
Flow Cytometry (FC)
Species Reactivity Mouse
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords . Product Images
mouse IgG2aκ (20 ng/lane), using 0.2ug/mL
of RevMAb clone RM107. This antibody only
reacts to nonreduced Mouse IgG2aκ.
reacts to the Fab region of mouse IgG2aκ; no cross
reactivity with IgG2aκ, IgG1, IgG3, IgM, IgA, IgE,
human IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM107 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
coated with different amounts of mouse IgG2aκ.
A serial dilution of RM107 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.
Western blot of nonreduced(-) and reduced(+)
ELISA of mouse immunoglobulins shows RM107
A titer ELISA of mouse IgG2aκ. The plate was