Western blot of nonreduced(-) and reduced(+) ELISA of mouse immunoglobulins shows RM108 A titer ELISA of mouse IgG2b. The plate was
Product Name Biotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody [RM108]
Product Description Biotinylated Rabbit monoclonal to Mouse IgG2b
Catalog No. 31-1004-02
Clone Name RM108
Product Data Sheet Biotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody Data Sheet RM108
Specificity RM108 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM108 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgG2b
Application Western Blot (WB) (stronger on nonreduced)
Flow Cytometry (FC)
Species Reactivity Mouse
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords . Product Images
mouse IgG2b (20ng/lane), using 0.2ug/mL of
RevMAb clone RM108. This antibody reacts to
nonreduced IgG2b (~150 kDa) much stronger
than the reduced γ2b form (~50 kDa).
reacts to the Fc region of mouse IgG2b; no cross
reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE,
human IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was
used as the primary antibody. An alkaline phosph-
atase conjugated anti-rabbit IgG as the secondary
coated with different amounts of mouse IgG2b.
A serial dilution of RM108 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.
Western blot of nonreduced(-) and reduced(+)
ELISA of mouse immunoglobulins shows RM108
A titer ELISA of mouse IgG2b. The plate was