Biotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone RM108

$260.00

Biotinylated Rabbit monoclonal to Mouse IgG2b

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Product Details
Product NameBiotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody [RM108]
Product DescriptionBiotinylated Rabbit monoclonal to Mouse IgG2b
Catalog No.31-1004-02
Clone NameRM108
Product Data SheetBiotin Anti-Mouse IgG2b Rabbit Monoclonal Antibody Data Sheet RM108
SpecificityRM108 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM108 has been engineered to eliminate Fc receptor binding.
ImmunogenMouse IgG2b
ApplicationWestern Blot (WB) (stronger on nonreduced)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateBiotin
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG2b (20ng/lane), using 0.2ug/mL of
RevMAb clone RM108. This antibody reacts to
nonreduced IgG2b (~150 kDa) much stronger
than the reduced γ2b form (~50 kDa).

ELISA

ELISA of mouse immunoglobulins shows RM108
reacts to the Fc region of mouse IgG2b; no cross
reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE,
human IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was
used as the primary antibody. An alkaline phosph-
atase conjugated anti-rabbit IgG as the secondary
antibody.

A

A titer ELISA of mouse IgG2b. The plate was
coated with different amounts of mouse IgG2b.
A serial dilution of RM108 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.


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