25(OH) Vitamin D Standard Curve: ELISA plate was coated with
Product Details
Product Name 25-OH Vitamin D3 Matched Antibody Pair [RM428/RMH04]
Product Description The 25-OH Vitamin D3 Matched Antibody Pair is specific to 25-OH Vitamin D3 and has been demonstrated through ELISA. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2. This product includes capture and detection antibodies.
Catalog No. 31-1315-MK
Clone Name RMH04 Anti-25-OH Vitamin D3 Rabbit/Human Chimeric Monoclonal Antibody
RM428 Biotinylated Anti-25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes Rabbit Monoclonal Antibody
Product Data Sheet 25-OH Vitamin D3 Matched Antibody Pair
Protocol 25-OH Vitamin D3 Sanwich ELISA Protocol
Specificity This antibody pair detects only 25-OH Vitamin D3. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2.
Application Sandwich ELISA, or other Sandwich Assays
Species Reactivity N/A
Conjugate RM428: Biotinylated
Intended Use Research Use Only
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Components (1) (2) Stable for 1 Year at -20.0°C from date of receipt.
Components 1) Capture Antibody: Clone RMH04, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS and 0.09% sodium azide;
2) Detection Antibody: Biotin Clone RM428, 50 μg at 1.0 mg/mL in 50% Glycerol/PBS and 0.09% sodium azide
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype RM428: Rabbit IgG
RMH04: Rabbit/Human Chimeric
Rabbit Fv/ Human IgG CH and Cκ
Keywords
Usage ELISA: Capture Antibody 50ng/well – 200ng/well;
Detection Antibody 0.5ug/mL – 2ug/mL;
.
Product Images
100 ng capture antibody RMH04 at concentration of 1ug/mL. A serial
dilution from 150 ng/mL was performed using 25(OH) vitamin D.
Biotin-labeled 25(OH) Vitamin D antibody, clone RM428 was
used as a detection antibody.
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