Detection of 5-Formylcytosines (5-fC) in linear denatured DNA (426 bp). Sandwich
Product Details
Product Name 5-Formylcytosines (5-fC) DNA Matched Antibody Pair
Product Description This antibody pair detects 5-Formylcytosines (5-fC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-caC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Catalog No. 31-1369-MK
Clone Name RM477 Anti-5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody; BSA Free
RM476 Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody
Product Data Sheet 5-Formylcytosines (5-fC) DNA Matched Antibody Pair Datasheet
Specificity This antibody pair detects 5-Formylcytosines (5-fC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-caC) in DNA.
Application Sandwich ELISA, or other Sandwich Assays
Species Reactivity Human
Conjugate RM476; Biotin
Intended Use Research Use Only
Related Products Anti-5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody, Clone RM477
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Components 1) Capture Antibody: Anti-5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody, Clone RM477, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins.
2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Usage ELISA: Capture Antibody 50ng/well – 200ng/well;
Detection Antibody 0.1ug/mL – 0.5ug/mL
Keywords
.
Product Images
ELISA using anti-Formylcytosines (5-fC) (clone RM477) as the capture antibody
(50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476)
as the detection antibody (0.2ug/mL), followed by an AP conjugated streptavidin.
Related Products
Matched Antibody Pair Kits
DNA Methylation
Matched Antibody Pair Kits
DNA Methylation
DNA Methylation
Matched Antibody Pair Kits
Matched Antibody Pair Kits
Matched Antibody Pair Kits
Matched Antibody Pair Kits
Matched Antibody Pair Kits
Matched Antibody Pair Kits
Matched Antibody Pair Kits