RM260 specifically reacts to Histone H2B acetylated Western Blot of (1) acid extracts of HeLa cells treated Immunocytochemistry of HeLa cells treated with
Product Details
Product Name Anti-Acetyl-Histone H2B (Lys23) Rabbit Monoclonal Antibody, Clone RM260
Product Description Rabbit monoclonal to Acetyl-Histone H2B (Lys23)
Catalog No. 31-1141-00
Clone Name RM260
Product Data Sheet Anti-Acetyl-Histone H2B (Lys23) Rabbit Monoclonal Antibody Data Sheet RM260
Specificity RM260 reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys23).
Application Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL – 2 µg/mL;
ICC: 0.5 µg/mL - 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
at Lysine 23 (K23ac). No cross reactivity with acetyl-
ated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12
(K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or
non-mordified Lysine 23 in histone H2B.
with sodium butyrate, (2) acid extracts of HeLa cells
untreated, and (3) recombinant Histone H2B, using
Acetyl-Histone H2B (Lys23) rabbit monoclonal antibody,
Clonc RM260, at 0.5 µg/mL.
sodium butyrate, using Acetyl-Histone H2B (Lys23)
rabbit monoclonal antibody, Clone RM260 (red). Actin
filaments have been labeled with fluorescein
phalloidin (green).
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