Anti-Androgen Receptor (AR-V7 specific) Rabbit Monoclonal Antibody, Clone RM7; ARv7 Splice Variant

$425.00

Rabbit monoclonal to Androgen Receptor (AR-V7 specific), ARv7 Splice Variant

Product Details
Product NameAnti-Androgen Receptor (AR-V7 specific) Rabbit Monoclonal Antibody [RM7]
Product DescriptionRabbit monoclonal to Androgen Receptor (AR-V7 specific), ARv7 Splice Variant
Catalog No.31-1109-00
Clone NameRM7
Product Data SheetAnti-Androgen Receptor (AR-V7 specific) Rabbit Monoclonal Antibody Data Sheet RM7
SpecificityRM7 reacts to androgen receptor splice variant 7 (AR-V7). No cross reactivity with wild type androgen receptor.
ImmunogenA peptide corresponding to AR splice variant 7
ApplicationImmunohistochemistry
Immunocytochemistry
Immunoprecipitation
Western Blot
Chromatin IP
Circulating Tumor Cells
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ul
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageImmunohistochemistry (IHC): 1:500 – 1:1000 dilution;
Immunocytochemistry (ICC): 1:100 – 1:1000 dilution;
Western Blot (WB): 1:500 – 1:1000 dilution.
References for ARv7 Splice Variant antibody [RM7]Sharp A et al. Androgen receptor splice variant-7 expression emerges with castration resistance in prostate cancer. J Clin Invest. (2018) 10.1172/JCI122819. IHC-P, IP, WB; Human. Read more (PubMed: 30334814)

Zhao P et al. Detection of androgen receptor (AR) and AR-V7 in small cell prostate carcinoma: Diagnostic and therapeutic implications. Asian J Urol. (2018), 10.1016/j.ajur.2018.09.003. WB, IHC-P; Human. Read more (Asian J Urol. AJUR244)

Russo JW et al. Downregulation of Dipeptidyl Peptidase 4 Accelerates Progression to Castration-Resistant Prostate Cancer. Cancer Res. (2018), 10.1158/0008-5472.CAN-18-0687. IHC-P; Human. Read more (PubMed: 30242112)

Yang RK et al. Expression pattern of androgen receptor and AR-V7 in androgen deprivation therapy naïve salivary duct carcinomas. Hum Pathol. (2018), 10.1016/j.humpath.2018.09.009. IHC-P; Human. Read more (PubMed: 30267779)

Lawrence MG et al. Patient-derived Models of Abiraterone- and Enzalutamide-resistant Prostate Cancer Reveal Sensitivity to Ribosome-directed Therapy. Eur Urol. (2018) 10.1016/j.eururo.2018.06.020. IHC-P; Human. Read more (PubMed: 30049486)

Kallio HML et al. Constitutively active androgen receptor splice variants AR-V3, AR-V7 and AR-V9 are co-expressed in castration-resistant prostate cancer metastases. Br J Cancer. (2018) 10.1038/s41416-018-0172-0. IHC-P; Human. Read more (PubMed: 29988112)

Zhao Pet al. Detection of androgen receptor (AR) and AR-V7 in small cell prostate carcinoma by immunohistochemistry. American Association for Cancer Research. (2018) 10.1158/1538-7445.AM2018-2640. IHC-P; Human. Read more (AACR 2018 Abstracts: 2640)

Miao L et al. Disrupting androgen receptor signaling induces Snail-mediated epithelial-mesenchymal plasticity in prostate cancer. Cancer Res. (2017) 10.1158/0008-5472.CAN-16-2169. WB; Human. Read more (PubMed: 28302679)

Moon SJ et al. DBC1 promotes castration-resistant prostate cancer by positively regulating DNA binding and stability of AR-V7. Oncogene (2018) 10.1158/0008-5472.CAN-16-2169. WB; Human. IP; Human. ChIP; Human. Read more (PubMed: 29249800)

Zhu Y et al. Novel Junction-specific and Quantifiable In Situ Detection of AR-V7 and its Clinical Correlates in Metastatic Castration-resistant Prostate Cancer. Eur Urol. (2017) 10.1016/j.eururo.2017.08.009. WB; Human. IHC-P; Human. Read more (PubMed: 28866255)

Luo J et al. Role of Androgen Receptor Variants in Prostate Cancer: Report from the 2017 Mission Androgen Receptor Variants Meeting. Eur Urol. (2017) 10.1016/j.eururo.2017.11.038. WB; Human. IHC-P; Human. ChIP; Human. Read more (PubMed: 29258679)
ProtocolsARv7 ICC Protocol

ARv7 IHC Protocol
Product Images
Western

Western Blot analysis of 22RV1, DU145, and PC-3 whole
cell lysates, using anti-AR-V7 rabbit monoclonal Antibody
Clone RM7, showed endogenous AR-V7 expressed in
22RV1 cells.

Immunocytochemical

Immunocytochemical staining of 22RV1 and PC-3 cells,
using anti-AR-V7 rabbit monoclonal Antibody Clone RM7
(red). Actin filaments have been labeled with fluorescein
phalloidin (green).

Immunohistochemical

Immunohistochemical staining of formalin fixed
and paraffin embedded 22RV1, DU145 sections.

Immunohistochemical staining of formalin fixed and
paraffin embedded (FFPE) bone metastasis prostate
cancer. Clinically validated to be Positive for ARv7.


Image courtesy of Dr. Stephen Plymate, University of
Washington, Washington, US

Immunohistochemical staining of formalin fixed and
paraffin embedded (FFPE) bone metastasis prostate
cancer. Clinically validated to be Negative for ARv7.


Image courtesy of Dr. Stephen Plymate, University of
Washington, Washington, US


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References

Sharp A et al. Androgen receptor splice variant-7 expression emerges with castration resistance in prostate cancer. J Clin Invest. (2018) 10.1172/JCI122819. IHC-P, IP, WB; Human. Read more (PubMed: 30334814)

Zhao P et al. Detection of androgen receptor (AR) and AR-V7 in small cell prostate carcinoma: Diagnostic and therapeutic implications. Asian J Urol. (2018), 10.1016/j.ajur.2018.09.003. WB, IHC-P; Human. Read more (Asian J Urol. AJUR244)

Russo JW et al. Downregulation of Dipeptidyl Peptidase 4 Accelerates Progression to Castration-Resistant Prostate Cancer. Cancer Res. (2018), 10.1158/0008-5472.CAN-18-0687. IHC-P; Human. Read more (PubMed: 30242112)

Yang RK et al. Expression pattern of androgen receptor and AR-V7 in androgen deprivation therapy naïve salivary duct carcinomas. Hum Pathol. (2018), 10.1016/j.humpath.2018.09.009. IHC-P; Human. Read more (PubMed: 30267779)

Lawrence MG et al. Patient-derived Models of Abiraterone- and Enzalutamide-resistant Prostate Cancer Reveal Sensitivity to Ribosome-directed Therapy. Eur Urol. (2018) 10.1016/j.eururo.2018.06.020. IHC-P; Human. Read more (PubMed: 30049486)

Kallio HML et al. Constitutively active androgen receptor splice variants AR-V3, AR-V7 and AR-V9 are co-expressed in castration-resistant prostate cancer metastases. Br J Cancer. (2018) 10.1038/s41416-018-0172-0. IHC-P; Human. Read more (PubMed: 29988112)

Zhao Pet al. Detection of androgen receptor (AR) and AR-V7 in small cell prostate carcinoma by immunohistochemistry. American Association for Cancer Research. (2018) 10.1158/1538-7445.AM2018-2640. IHC-P; Human. Read more (AACR 2018 Abstracts: 2640)

Miao L et al. Disrupting androgen receptor signaling induces Snail-mediated epithelial-mesenchymal plasticity in prostate cancer. Cancer Res. (2017) 10.1158/0008-5472.CAN-16-2169. WB; Human. Read more (PubMed: 28302679)

Moon SJ et al. DBC1 promotes castration-resistant prostate cancer by positively regulating DNA binding and stability of AR-V7. Oncogene (2018) 10.1158/0008-5472.CAN-16-2169. WB; Human. IP; Human. ChIP; Human. Read more (PubMed: 29249800)

Zhu Y et al. Novel Junction-specific and Quantifiable In Situ Detection of AR-V7 and its Clinical Correlates in Metastatic Castration-resistant Prostate Cancer. Eur Urol. (2017) 10.1016/j.eururo.2017.08.009. WB; Human. IHC-P; Human. Read more (PubMed: 28866255)

Luo J et al. Role of Androgen Receptor Variants in Prostate Cancer: Report from the 2017 Mission Androgen Receptor Variants Meeting. Eur Urol. (2017) 10.1016/j.eururo.2017.11.038. WB; Human. IHC-P; Human. ChIP; Human. Read more (PubMed: 29258679)