Immunohistochemical staining of formalin fixed and paraffin Immunocytochemical staining of HeLa cells, using Western blot of A431 cells, using anti-β-Actin RevMAb
Product Name Anti-β-Actin Rabbit Monoclonal Antibody [RM112]
Description Rabbit monoclonal to β-Actin (beta-actin)
Catalog No. 31-1013-00
Clone Name RM112
Product Data Sheet Anti-Beta Actin Rabbit Monoclonal Antibody Data Sheet RM112
Specificity RM112 reacts to β-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.
Immunogen A peptide corresponding to the N-terminus of β-actin
Application Western Blot (WB)
Flow Cytometry (FC)
Species Reactivity All Species
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot (WB): 1:1000 dilution;
Immunoprecipitation (IP): 1:200 dilution;
Chromatin IP (ChIP): 1:200 dilution;
Immunocytochemistry (ICC): 1:200 dilution;
Immunohistochemistry (IHC): 1:200-1:1000 dilution.
Keywords References for β-Actin Rabbit Monoclonal Antibody [RM112] Jevtić P et al. The nucleoporin ELYS regulates nuclear size by controlling NPC number and nuclear import capacity. EMBO Rep. (2019), 10.15252/embr.201847283. WB; Human. Read more (PubMed: 31085625)
Wang G et al. Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. Elife. (2019), 10.7554/eLife.38730. WB; Human. Read more (PubMed: 31042147)
Mukherjee RN et al. Reticulon 4a promotes exocytosis in mammalian cells. Elife. (2019), 10.1091/mbc.E19-03-0159. WB; Human. Read more (PubMed: 31318320)
embedded human tonsil tissue using Anti-beta actin
Rabbit Monoclonal Antibody (Clone RM112) at a 1:1000 dilution.
anti-β-Actin RevMAb Clone RM112 at 1/200 dilution (red).
Nuclei have been labeled with DAPI (blue).
Clone RM112 at a 1/1000 dilution, showed a band of
β-Actin at the predicted MW (42 kDa).
Immunohistochemical staining of formalin fixed and paraffin
Immunocytochemical staining of HeLa cells, using
Western blot of A431 cells, using anti-β-Actin RevMAb