Immunohistochemical staining of formalin fixed and ELISA showing RM127 reacts only to the lambda A titer ELISA using RM127. The plate was coated ELISA showing RM127 does not react to monkey
Product Name Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody [RM127]
Description Rabbit monoclonal to Human Lambda Light Chain
Catalog No. 31-1029-00
Clone Name RM127
Product Data Sheet Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody Data Sheet RM127
Specificity RM127 reacts to the lambda light chain of human immunoglobulins, and does not react to the kappa light chain. RM127 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG
Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.05ug/mL – 0.2ug/mL;
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL;
Flow Cytometry (FC): 0.2ug/mL-2ug/mL.
Keywords References for Human Ig Lambda Light Chain antibody [RM127] Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)
paraffin embedded human tonsil tissue section using
anti-human Ig lambda light chain rabbit monoclonal
antibody (Clone RM127).
light chain of human immunoglobulins, and not to
the kappa light chain, mouse IgG, rat IgG, or goat IgG.
with different amounts of human IgG3λ. A serial
dilution of RM127 was used as the primary antibody,
followed by an alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM127 and a monkey IgG binding
antibody (positive control) was used as the detection
Immunohistochemical staining of formalin fixed and
ELISA showing RM127 reacts only to the lambda
A titer ELISA using RM127. The plate was coated
ELISA showing RM127 does not react to monkey