Immunohistochemical staining of Giant Cell Bone Image courtesy of Adrienne Flanagan, Department IHC of clinically validated FFPE Glioblastoma tumor Image courtesy of Dr. Sebastian Brandner, UCL Immunostaining of brain tumor tissue sections Image courtesy of Sebastian Brandner MD, Division Immunohistochemical staining of formalin fixed Image courtesy of Adrienne Flanagan, Department Immunohistochemical staining of Giant Cell Bone Tumor (GCBT) Image courtesy of Hidetaka Yamamoto, MD, PhD,
Product Details
Product Name Histone H3 Mutation (Mutated K4M, K9M, K27M, K36M) + H3.3 Mutation (Mutated G34W, G34R, G34V) Antibody Panel, Clone RM191, RM192, RM193, RM363, RM263, RM240, RM307
Product Description RevMAb's Antibody Panel of rabbit monoclonal recombinant antibodies to Histone H3.3 G34W, Histone H3.3 G34R, Histone H3.3 G34V, Histone H3 K4M, Histone H3 K9M, Histone H3 K27M, and Histone H3 K36M
Catalog No. 31-1327-MP
Clone Name RM191, RM192, RM193, RM363, RM263, RM240, RM307
Product Data Sheet Histone H3.3/ H3 Antibody Panel Data Sheet
Specificity Histone H3.3/ H3 Antibody Panel reacts to Histone H3 K27M, K36M, K4M, K9M, along with H3.3 G34W, G34R, and G34V mutants. No cross reactivity with wild type Histone H3.
Immunogen A peptide corresponding to Histone H3 K27M, K36M, K4M, K9M, along with H3.3 G34W, G34R, and G34V mutants individually.
Application Western Blot
ELISA
Immunohistochemistry
Species Reactivity All
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 25 µL each
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1:125 – 1:1000;
ELISA: 1:125 – 1:1000;
IHC: 1:100 – 1:500.
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Product Images
Tumor (GCBT) tissue sections using Anti-Histone
H3.3 G34W antibody, Clone RM263.
of Histopathology, RONH, Stanmore, Middlesex, UK
tissues with H3.3 G34R expression, using anti-histone
H3.3 G34R rabbit monoclonal antibody, Clone RM240.
Clinically validated to be Positive for H3.3 G34R Mutation.
Institute of Neurology, London, United Kingdom
using both EDTA and Citrate pretreatment methods.
Clinically validated to be Positive for the H3.3 K27M
Mutation by Sanger sequencing.
of Neuropathology and Dept. of Neurodegenerative Disease,
UCL Institute of Neurology, London, UK
paraffin embedded (FFPE) Chondroblastoma tumor
cell tissue sections using RM193 Anti-Histone H3
K36M mAb at a 1:400 dilution.
of Histopathology, RONH, Stanmore, Middlesex, UK
tissue sections using Anti-Histone H3.3 G34V antibody, Clone RM307.
Kyushu University, Fukuoka, Japan
Related Products
Histone Mutant Panels
Histone H3.3 Mutation Antibody Panel (Mutated G34W, G34R, G34V), Clone RM263, RM240, RM307
Histone Mutant Panels
Histone H3 Mutation Antibody Panel (Mutated K4M, K9M, K27M, K36M), Clone RM363, RM191, RM192, RM193