Western Blot of (1) H2A recombinant protein and RM445 specifically reacts to Histone H2AX acetylated at Lysine 5 (K5ac).
Product Name Anti-Acetyl-Histone H2A.X (Lys5) Rabbit Monoclonal Antibody [RM445]
Product Description Rabbit monoclonal to Acetyl-Histone H2A.X (Lys5)
Catalog No. 31-1336-00
Clone Name RM445
Product Data Sheet Acetyl-Histone H2A.X (Lys5) Rabbit Monoclonal Antibody Data Sheet RM445
Specificity RM445 reacts to Histone H2A.X acetylated at Lysine 5 (K5ac). No cross reactivity with non-modified Lysine 5 or other acetylated Lysines in histone H2A.
Immunogen A peptide corresponding to acetyl-Histone H2A.X (Lys5).
Application Western Blot
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 0.04 ug/mL – 0.2 ug/mL;
Multiplex: 0.2 ug/mL - 1 ug/mL.
Keywords . Product Images
(2) acid extracts of HeLa cell lysate, using
acetyl-Histone H2A.X (Lys5) rabbit monoclonal
antibody (RM445) at 0.04 ug/mL.
No or limited cross reactivity with acetylated Lysine 9 (K9ac), Lysine 36 (K36ac)
in Histone H2AX, Lysine 4 (K4ac), Lysine 7 (K7ac), Lysine 11 (K11ac), Lysine 13 (K13ac)
in Histone H2AZ, or non-modified Asparagine 19 (N19), Lysine 36 (K36) in Histone H2AX.
Western Blot of (1) H2A recombinant protein and
RM445 specifically reacts to Histone H2AX acetylated at Lysine 5 (K5ac).