Sandwich ELISA using RM122 as the capture Sandwich ELISA, using RevMAb human IgE matched Sandwich ELISA, using RevMAb human IgE matched antibody pair,
Product Name Human IgE ELISA Antibody Pair Kit
Product Description The Human IgE Matched Antibody Pairs is specific to Human IgE and has been demonstrated through ELISA. It does not cross-react to IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), or mouse, rat or goat immunoglobulin. The kit can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data. This kit includes capture and detection antibodies and sample diluent buffer.
Catalog No. 31-1024-MK
Clone Name RM122 Anti-Human IgE Rabbit Monoclonal Antibody
RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody
Product Data Sheet Human IgE Matched Antibody Pair
Specificity The antibody pair detects only human IgE. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses.
Application Sandwich ELISA, or other Sandwich Assays
Species Reactivity Human
Conjugate RM129: Biotin
Intended Use Research Use Only
.Protocol Sandwich ELISA Protocol
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Components 1) Capture Antibody: Anti-Human IgE Rabbit Monoclonal antibody, clone RM122, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Keywords Usage ELISA: Capture Antibody 50ng/well – 200ng/well;
Detection Antibody 0.1ug/mL – 0.5ug/mL
. Product Images
antibody (100ng/well), and Biotinylated anti-human
light chains (k+l) antibody RM129 (0.2ug/mL) as the
detection antibody, followed by an alkaline phosphatase
antibody pair (capture: RM122; detection: Biotin-RM129),
shows species reactivity to human only, and shows no
cross-reactivity to monkey (Cyno or Rhesus), mouse IgE,
rat IgE, or goat IgE.
shows no cross- reactivity with Human IgG, IgM, IgA, or IgD.
The plate was coated with human IgE capture antibody (50ng/well).
Different immunoglobulin samples (0.2 ug/mL) were added and
biotinylated RM129 (0.2ug/mL) was used as detection antibody,
followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA using RM122 as the capture
Sandwich ELISA, using RevMAb human IgE matched
Sandwich ELISA, using RevMAb human IgE matched antibody pair,