Sandwich ELISA using RM121 as the capture Sandwich ELISA, using RevMAb human IgM matched Sandwich ELISA, using RevMAb human IgM matched antibody
Product Name Human IgM ELISA Antibody Pair Kit
Product Description The Human IgM Matched Antibody Pairs is specific to Human IgM and has been demonstrated through ELISA. It does not cross-react to IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), or mouse, rat or goat immunoglobulin. The kit can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data. This kit includes capture and detection antibodies and sample diluent buffer.
Catalog No. 31-1023-MK
Clone Name RM121 Anti-Human IgM Rabbit Monoclonal Antibody
RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody
Product Data Sheet Human IgM Matched Antibody Pair
Protocol Sandwich ELISA Protocol
Specificity The antibody pair detects only human IgM. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses.
Application Sandwich ELISA, or other Sandwich Assays
Species Reactivity Human
Conjugate RM129: Biotin
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Components 1) Capture Antibody: Anti-Human IgM Rabbit Monoclonal antibody, clone RM121, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Keywords Usage ELISA: Capture Antibody 50ng/well – 200ng/well;
Detection Antibody 0.1ug/mL – 0.5ug/mL
. Product Images
antibody (100ng/well), and Biotinylated anti-human light
chains (k+l) antibody RM129 (0.2ug/mL) as the detection
antibody, followed by an alkaline phosphatase
antibody pair (capture: RM121; detection: Biotin-RM129),
shows species reactivity to human IgM only, and
shows no cross-reactivity to monkey (Cyno or
Rhesus), mouse IgM, rat IgM, or goat.
pair, shows no cross- reactivity with Human IgG, IgE, IgA, or IgD.
The plate was coated with human IgM capture antibody (50ng/well).
Different immunoglobulin samples (0.2 ug/mL) were added
and biotinylated RM129 (0.2ug/mL) was used as detection antibody,
followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA using RM121 as the capture
Sandwich ELISA, using RevMAb human IgM matched
Sandwich ELISA, using RevMAb human IgM matched antibody