Anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody, Clone RM205; H4K20ac, Histone H4 (K20 acetylation)

$155.00$360.00

Rabbit monoclonal to Acetyl-Histone H4 (Lys20), H4K20ac, Histone H4 (K20 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody [RM205]
Product DescriptionRabbit monoclonal to Acetylated Histone H4 Lysine 20 (K20ac), H4K20ac, Histone H4 (K20 acetylation)
Catalog No.31-1084-00
Clone NameRM205
Product Data SheetAnti-Acetyl-Histone H4 Lys20 Rabbit Monoclonal Antibody Data Sheet RM205
SpecificityRM205 reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with other acetylated Lysines in Histone H4.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H4 (Lys20).
ApplicationWestern Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 1 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL;
Immunocytochemistry: 1 ug/mL- 2 ug/mL.
Keywords
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Product Images
Western

Western Blot of (1) acid extracts of HeLa cells;
(2) recombinant Histone H4. Using RM205 at 1 ug/mL,
showed a band of Histone H4 acetylated at Lysine 20
in HeLa cells.

RM205

RM205 specifically reacts to Histone H4 acetylated
at Lysine 20 (K20ac). No cross reactivity with
unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5
(K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16
(K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in
Histone H4.

Immunocytochemical

Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H4 (Lys20) Rabbit Monoclonal Antibody (clone
RM205) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).


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