Western Blot of acid extracts of HeLa cells non-treated RM169 specifically reacts to Histone H3 acetylated at Lysine 23 Immunocytochemical staining of HeLa cells treated
Product Name Anti-Acetyl-Histone H3 (Lys523) Rabbit Monoclonal Antibody [RM169]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 23 (K23ac), H3K23ac, Histone H3 (K23 acetylation)
Catalog No. 31-1087-00
Clone Name RM169
Product Data Sheet Anti-Acetyl-Histone H3 Lys23 Rabbit Monoclonal Antibody Data Sheet RM169
Specificity RM169 reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys23).
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.2 ug/mL – 1 ug/mL;
Immunocytochemistry: 1 ug/mL- 2 ug/mL.
Keywords References for Acetyl-Histone H3 (Lys23) antibody [RM169] Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)
(1) or treated with sodium butyrate (2), and recombinant
histone H3.3 (3), using RM169 at 1 ug/mL, showed a
band of histone H3 acetylated at Lysine 23 in treated HeLa.
(K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl),
acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),
Lysine 18 (K18ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
with sodium butyrate, using anti-Acetyl-Histone
H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone
RM221) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).
Western Blot of acid extracts of HeLa cells non-treated
RM169 specifically reacts to Histone H3 acetylated at Lysine 23
Immunocytochemical staining of HeLa cells treated