Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone RM186; Histone H3 (Unmodified Lys4)

$155.00$360.00

Rabbit Monoclonal to Histone H3 (Unmodified Lys4); Histone H3 (Unmodified Lysine 4)

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Product Details
Product NameAnti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody [RM186]
Product DescriptionRabbit Monoclonal to Histone H3 (Unmodified Lys4); Histone H3 (Unmodified Lysine 4)
Catalog No.31-1086-00
Clone NameRM186
Product Data SheetAnti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody Data Sheet RM186
SpecificityRM186 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.
ImmunogenAn unmodified peptide corresponding to the N-terminus of Histone H3.
ApplicationWestern Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 1 ug/mL – 2 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.



Keywords
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Product Images
RM186

RM186 specifically recognizes Histone H3 unmodified
at Lys4 and does not recognize acetylated, mono-
methylated, dimethylated, or trimethylated Lys4. The
antibody binding specificity allows for modifications of
Arg2 or Thr3 in histone H3.

Western

Western Blot of acid extracts of HeLa cells treated with
sodium butyrate (HeLa+NaBu), treated with Nocodazole
(HeLa+Noc), or non-treated; and recombinant histone
H3.1 protein, using RM186 at 1 ug/mL, showed a band
of histone H3 with unmodified Lys4.

Immunocytochemical

Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H3 (Unmodified Lys4) Rabbit Monoclonal Antibody (clone
RM186) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).


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