RM186 specifically recognizes Histone H3 unmodified Western Blot of acid extracts of HeLa cells treated with Immunocytochemical staining of HeLa cells treated
Product Name Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody [RM186]
Product Description Rabbit Monoclonal to Histone H3 (Unmodified Lys4); Histone H3 (Unmodified Lysine 4)
Catalog No. 31-1086-00
Clone Name RM186
Product Data Sheet Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody Data Sheet RM186
Specificity RM186 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.
Immunogen An unmodified peptide corresponding to the N-terminus of Histone H3.
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1 ug/mL – 2 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
Keywords . Product Images
at Lys4 and does not recognize acetylated, mono-
methylated, dimethylated, or trimethylated Lys4. The
antibody binding specificity allows for modifications of
Arg2 or Thr3 in histone H3.
sodium butyrate (HeLa+NaBu), treated with Nocodazole
(HeLa+Noc), or non-treated; and recombinant histone
H3.1 protein, using RM186 at 1 ug/mL, showed a band
of histone H3 with unmodified Lys4.
with sodium butyrate, using anti-Acetyl-Histone
H3 (Unmodified Lys4) Rabbit Monoclonal Antibody (clone
RM186) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).
RM186 specifically recognizes Histone H3 unmodified
Western Blot of acid extracts of HeLa cells treated with
Immunocytochemical staining of HeLa cells treated