Anti-Human IgA (α1 & α2) Rabbit Monoclonal Antibody, Clone RM128

$245.00

Rabbit monoclonal to Human IgA (α1 & α2)

SKU: 31-1030-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human IgA (α1& α2) Rabbit Monoclonal Antibody [RM128]
Product DescriptionRabbit monoclonal to Human IgA (α1& α2)
Catalog No.31-1030-00
Clone NameRM128
Product Data SheetAnti-Human IgA Rabbit Monoclonal Antibody Data Sheet RM128
SpecificityRM128 reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
ImmunogenHuman IgA
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.1ug/mL-1ug/mL.
Keywords
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Product Images
Immunohistochemistry

Immunohistochemistry of Human Lymphoid Tissue using
anti-Human IgA antibody RM128.

Sandwich

Sandwich ELISA using RM128 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

Sandwich

Sandwich ELISA using RM128 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

ELISA

ELISA of human immunoglobulins shows RM128
reacts to both Human IgA1 & IgA2. No cross
reactivity with Human IgG, IgM, IgD, or IgE.
The plate was coated with 50 ng/well of different
immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM128 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.

A

A titer ELISA using RM128. The plate was coated
with different amounts of human IgA. A serial dilution
of RM128 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.


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