Detection of human IgG1 in monkey serum, using ELISA showing RM129 reacts only to kappa and ELISA showing RM129 does not react to monkey A titer Sandwich ELISA using Biotinylated RM129
Product Name Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody [RM129]
Product Description Biotinylated Rabbit monoclonal to Human Immunoglobulin Light Chains
Catalog No. 32-1031-02
Clone Name RM129
Product Data Sheet Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody Data Sheet RM129
Specificity RM129 reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG
Flow Cytometry (FC)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.02ug/mL – 0.25ug/mL;
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL;
Flow Cytometry (FC): 0.5ug/mL-2ug/mL.
Keywords . Product Images
RM117 (capture) and biotin-RM129 (detection)
as a Sandwich ELISA pair. HRP conjugated
streptavidin and TMB were used to yield the
lambda light chain of all human immunoglobulins,
not to mouse IgG, rat IgG, or goat IgG.
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM129 and a monkey IgG binding
antibody (positive control) was used as the detection
as the detection antibody. The plate, coated
with the capture antibody anti-human IgG RM116,
was loaded with different amounts of human IgG.
A serial dilution of Biotin RM129 was used as
the detection antibody, followed by an alkaline
phosphatase conjugated streptavidin.
Detection of human IgG1 in monkey serum, using
ELISA showing RM129 reacts only to kappa and
ELISA showing RM129 does not react to monkey
A titer Sandwich ELISA using Biotinylated RM129