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Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122

$310.00

Rabbit monoclonal to Human IgE

SKU: 31-1024-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human IgE Rabbit Monoclonal Antibody [RM122]
Product DescriptionRabbit monoclonal to Human IgE
Catalog No.31-1024-00
Clone NameRM122
Product Data SheetAnti-Human IgE Rabbit Monoclonal Antibody Data Sheet RM122
SpecificityRM122 reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.
ImmunogenHuman IgE
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 10ng/well – 100ng/well (for Capture);
0.01ug/mL – 0.1ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
Sandwich

Sandwich ELISA using RM122 as the capture antibody
 (25ng/well), and Biotinylated anti-human light chains
 ( κ+ λ) antibody RM129 as the detection antibody, followed
 by an alkaline phosphatase conjugated streptavidin.

ELISA

ELISA of human immunoglobulins shows RM122
reacts only to human IgEλ from human myeloma
plasma and the IgE from human plasma. No cross
reactivity with Human IgG, IgM, IgD, or IgA. The
plate was coated with 50 ng/well of different
immunoglobulins. 200 ng/mL, 50 ng/mL or 10 ng/mL
of RM122 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.

A

A titer ELISA using RM122. The plate was coated
with different amounts of human IgE. A serial dilution
 of RM122 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.


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