Sandwich ELISA using RM122 as the capture antibody ELISA of human immunoglobulins shows RM122 A titer ELISA using RM122. The plate was coated
Product Details
Product Name Anti-Human IgE Rabbit Monoclonal Antibody [RM122]
Product Description Rabbit monoclonal to Human IgE
Catalog No. 31-1024-00
Clone Name RM122
Product Data Sheet Anti-Human IgE Rabbit Monoclonal Antibody Data Sheet RM122
Specificity RM122 reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.
Immunogen Human IgE
Application ELISA
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 10ng/well – 100ng/well (for Capture);
0.01ug/mL – 0.1ug/mL (for Detection);
Keywords
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Product Images
(25ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
reacts only to human IgEλ from human myeloma
plasma and the IgE from human plasma. No cross
reactivity with Human IgG, IgM, IgD, or IgA. The
plate was coated with 50 ng/well of different
immunoglobulins. 200 ng/mL, 50 ng/mL or 10 ng/mL
of RM122 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
with different amounts of human IgE. A serial dilution
of RM122 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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