Detection of human IgG1 in monkey serum, using Sandwich ELISA using RM117 as the capture ELISA showing RM117 reacts only to human IgG1, A titer ELISA using RM117. The plate was coated ELISA showing RM117 does not react to monkey Immunohistochemical staining of FFPE Tonsil tissue Image courtesy of Shaomin Hu, MD, PhD,
Product Name Anti-Human IgG1 Rabbit Monoclonal Antibody [RM117]
Product Description Rabbit monoclonal to Human IgG1
Catalog No. 31-1019-00
Clone Name RM117
Product Data Sheet Anti-Human IgG1 Rabbit Monoclonal Antibody Data Sheet RM117
Specificity RM117 reacts to the heavy chain of human IgG1. RM117 does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM117 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen Peptide corresponding to the hinge region of Human IgG1
Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
References for Human IgG1 antibody [RM117] Harel E et al. Further Evidence That the Soluble Urokinase Plasminogen Activator Receptor Does Not Directly Injure Mice or Human Podocytes. J Transplantation. (2020), 10.1097/TP.0000000000002930. IHC; Human. Read more (PubMed: 31449183)
Sulka KB et al. Lyme Disease-Associated IgG4 Autoantibodies Correlate with Synovial Pathology in Antibiotic-Refractory Lyme Arthritis. Arthritis Rheumatol. (2018) 10.1002/art.40566. IHC-P; Human. Read more (PubMed: 29790305)
. Product Images
RM117 (capture) and biotin-RM129 (detection)
as a Sandwich ELISA pair. HRP conjugated
streptavidin and TMB were used to yield the
antibody, and biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
and not to any other IgG subclasses (IgG2, IgG3,
or IgG4), and no cross reactivity to IgM, IgA, IgD,
IgE, mouse IgG, rat IgG, or goat IgG.
with different amounts of human IgG1. A serial
dilution of RM117 was used as the primary antibody,
followed by an alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM117 and a monkey IgG binding
antibody (positive control) was used as the detection
sections using Anti-Human IgG1 antibody RM117
at a 1:15,000 dilution.
Department of Anatomic and Clinical Pathology.
Montefiore Medical Center
Albert Einstein College of Medicine.
Bronx, New York, USA
Detection of human IgG1 in monkey serum, using
Sandwich ELISA using RM117 as the capture
ELISA showing RM117 reacts only to human IgG1,
A titer ELISA using RM117. The plate was coated
ELISA showing RM117 does not react to monkey
Immunohistochemical staining of FFPE Tonsil tissue
Image courtesy of Shaomin Hu, MD, PhD,