Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121

$230.00

Rabbit monoclonal to Human IgM

SKU: 31-1023-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human IgM Rabbit Monoclonal Antibody [RM121]
Product DescriptionRabbit monoclonal to Human IgM
Catalog No.31-1023-00
Clone NameRM121
Product Data SheetAnti-Human IgM Rabbit Monoclonal Antibody Data Sheet RM121
SpecificityRM121 reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
ImmunogenHuman IgM
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
Sandwich

Sandwich ELISA using RM121 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

Sandwich

Sandwich ELISA using RM121 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

ELISA

ELISA of human immunoglobulins shows RM121
reacts only to human IgM. No cross reactivity
with Human IgG, IgE, IgD, or IgA. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL of RM121 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.

A

A titer ELISA using RM121. The plate was coated
with different amounts of human IgM. A serial dilution
of RM121 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.


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