Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129

$275.00

Biotinylated Rabbit monoclonal to Human Immunoglobulin Light Chains

SKU: 32-1031-02. Categories: , , . Tags: , .

Product Details
Product NameBiotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody [RM129]
Product DescriptionBiotinylated Rabbit monoclonal to Human Immunoglobulin Light Chains
Catalog No.32-1031-02
Clone NameRM129
Product Data SheetBiotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody Data Sheet RM129
SpecificityRM129 reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
ImmunogenHuman IgG
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateBiotin
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.02ug/mL – 0.25ug/mL;
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
Detection

Detection of human IgG1 in monkey serum, using
RM117 (capture) and biotin-RM129 (detection)
as a Sandwich ELISA pair. HRP conjugated
streptavidin and TMB were used to yield the
colorimetric reaction.

ELISA

ELISA showing RM129 reacts only to kappa and
lambda light chain of all human immunoglobulins,
not to mouse IgG, rat IgG, or goat IgG.

ELISA

ELISA showing RM129 reacts only to kappa and
lambda light chain of all human immunoglobulins,
not to mouse IgG, rat IgG, or goat IgG.

ELISA

ELISA showing RM129 does not react to monkey
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM129 and a monkey IgG binding
antibody (positive control) was used as the detection
antibody.

A

A titer Sandwich ELISA using Biotinylated RM129
as the detection antibody. The plate, coated
with the capture antibody anti-human IgG RM116,
was loaded with different amounts of human IgG.
A serial dilution of Biotin RM129 was used as
the detection antibody, followed by an alkaline
phosphatase conjugated streptavidin.


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