Anti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM322

$155.00$360.00

Rabbit monoclonal to Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14)

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Product Details
Product NameAnti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [RM385]
Product DescriptionRabbit monoclonal to Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14)
Catalog No.31-1207-00
Clone NameRM322
Product Data SheetAnti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody Data Sheet RM322
SpecificityRM322 reacts to Histone H3 only when modified by both methylation at lysine 9 and acetylation at lysine 14 (K9me2/K14ac). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), trimethylated Lysine 9 (K9me3), or acetylated Lysine 9/14 only, in histone H3.
ImmunogenA dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys9) and an acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14)
ApplicationWestern Blot (WB)
Multiplex
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100 ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWB: 0.1 ug/mL – 1 ug/mL;
Multiplex: 0.01 ug/mL – 1 ug/mL.
Keywords
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Product Images
RM322

RM322 specifically reacts to Histone H3 only when
modified at both dimethyl-lysine 9 and acetyl-lysine
14 (K9me2/K14ac). No cross reactivity with non-
modified Lysine 9/14, methylated Lysine 9 (K9me1,
k9me2, k9me3) ONLY, acetylated Lysine 9/14 ONLY,
or methylated Lysine 14 (K14me2) ONLY, in Histone H3.

Western

Western Blot of HeLa cells (1) and recombinant
histone H3.3 (2) using RM322 at 0.5 ug/mL.


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