RM322 specifically reacts to Histone H3 only when Western Blot of HeLa cells (1) and recombinant
Product Details
Product Name Anti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [RM385]
Product Description Rabbit monoclonal to Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14)
Catalog No. 31-1207-00
Clone Name RM322
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody Data Sheet RM322
Specificity RM322 reacts to Histone H3 only when modified by both methylation at lysine 9 and acetylation at lysine 14 (K9me2/K14ac). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), trimethylated Lysine 9 (K9me3), or acetylated Lysine 9/14 only, in histone H3.
Immunogen A dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys9) and an acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14)
Application Western Blot (WB)
Multiplex
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 0.1 ug/mL – 1 ug/mL;
Multiplex: 0.01 ug/mL – 1 ug/mL.
Keywords
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Product Images
modified at both dimethyl-lysine 9 and acetyl-lysine
14 (K9me2/K14ac). No cross reactivity with non-
modified Lysine 9/14, methylated Lysine 9 (K9me1,
k9me2, k9me3) ONLY, acetylated Lysine 9/14 ONLY,
or methylated Lysine 14 (K14me2) ONLY, in Histone H3.
histone H3.3 (2) using RM322 at 0.5 ug/mL.
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