Anti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM389; H3K9me3, Histone H3 (trimethyl K9)


Rabbit monoclonal to Trimethylated Histone H3 Lysine 9, H3K9me3, Histone H3 (trimethyl K9)

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Product Details
Product NameAnti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM389]
Product DescriptionRabbit monoclonal to Trimethylated Histone H3 Lysine 9, H3K9me3, Histone H3 (trimethyl K9)
Catalog No.31-1275-00
Clone NameRM389
Product Data SheetAnti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM389
SpecificityRM389 reacts to Histone H3 trimethylated at Lysine 9 (K9me3). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), or other methylations in histone H3.
ImmunogenA trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys9).
ApplicationWestern Blot (WB)
Species ReactivityHuman
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt.
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL-2 ug/mL;
ELISA: 1 ug/mL - 10 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL.

Product Images

Western Blot of recombinant histone H3.3 (1) and
acid extracts of HeLa cells (2), using RM389 at 0.5
ug/mL, showed a band of histone H3 trimethylated
at Lysine 9 (K9me3) in HeLa cells.

ELISA of Designer Nucleosomes (dNucs) (Recombinant
Human Nucleosome with H3 K4, 9, 27, 36 and H4 K20
Mono-, Di-, and Tri-Methylation), using anti-H3K9me3
rabbit monoclonal antibody RM389 (0.5 μg/mL).

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Size Format

25ug – $155, 100ug – $360

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