Western Blot of recombinant histone H3.3 (1) and ELISA of Designer Nucleosomes (dNucs) (Recombinant
Product Details
Product Name Anti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM389]
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 9, H3K9me3, Histone H3 (trimethyl K9)
Catalog No. 31-1275-00
Clone Name RM389
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM389
Specificity RM389 reacts to Histone H3 trimethylated at Lysine 9 (K9me3). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys9).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL-2 ug/mL;
ELISA: 1 ug/mL - 10 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL.
Keywords
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Product Images
acid extracts of HeLa cells (2), using RM389 at 0.5
ug/mL, showed a band of histone H3 trimethylated
at Lysine 9 (K9me3) in HeLa cells.
Human Nucleosome with H3 K4, 9, 27, 36 and H4 K20
Mono-, Di-, and Tri-Methylation), using anti-H3K9me3
rabbit monoclonal antibody RM389 (0.5 μg/mL).
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| Size Format | 25ug – $179, 100ug – $399 |
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