Western Blot of recombinant histone H3.3 (1) and ELISA of Designer Nucleosomes (dNucs) (Recombinant
Product Name Anti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM389]
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 9, H3K9me3, Histone H3 (trimethyl K9)
Catalog No. 31-1275-00
Clone Name RM389
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM389
Specificity RM389 reacts to Histone H3 trimethylated at Lysine 9 (K9me3). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys9).
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL-2 ug/mL;
ELISA: 1 ug/mL - 10 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL.
Keywords . Product Images
acid extracts of HeLa cells (2), using RM389 at 0.5
ug/mL, showed a band of histone H3 trimethylated
at Lysine 9 (K9me3) in HeLa cells.
Human Nucleosome with H3 K4, 9, 27, 36 and H4 K20
Mono-, Di-, and Tri-Methylation), using anti-H3K9me3
rabbit monoclonal antibody RM389 (0.5 μg/mL).
Western Blot of recombinant histone H3.3 (1) and
ELISA of Designer Nucleosomes (dNucs) (Recombinant