Anti-N6-Methyladenosine Rabbit Monoclonal Antibody, Clone RM362; (m6A)

Name: Anti-N6-Methyladenosine Rabbit Monoclonal Antibody, Clone RM362; (m6A)
SKU: 31-1248-00
Price: 329.00 USD
Availability: InStock

$329.00

Rabbit monoclonal to N6-Methyladenosine (m6A)

SKU: 31-1248-00 Categories: DNA Methylation, Epigenetics, Primary Antibodies, Rabbit Monoclonal Tags: 31-1248-00, 31124800

Description
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Product Details
Product Name	Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody [RM362]
Product Description	Rabbit monoclonal to N6-Methyladenosine (m6A)
Catalog No.	31-1248-00
Clone Name	RM362
Product Data Sheet	Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody Data Sheet RM362
Specificity	RM362 reacts to N6-Methyladenosine (m6A) in both RNA and DNA. No cross reactivity with non-methylated adenosine.
Immunogen	BSA-conjugated N6-Methyladenosine
Application	Dot Blot ELISA Methylated RNA Immunoprecipitation
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	DotBlot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.1 ug/mL – 3 ug/mL; MeRIP: 0.2 ug/mL – 5 ug/mL
Keywords
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Product Images
	Dot blot of Lambda DNA without or with m6A, using anti-m6A antibody (RM362). The membrane was pre-spotted with 5, and 0.5 ng/dot of lambda DNA. m6A-free DNA was isolated from bacteriophage lambda grown in an E. coli host-deficient in adenine methylase (dam-).
	MeRIP was performed using M6A and control RNA from NEB (E1610). Antibodies (Anti-m6A RM362 and Anti-PEG RM105) were mixed for a short time, with Protein A beads before the addition of m6A labelled and non- labelled RNA (in equal amounts). Captured RNA was then washed, eluted and run through RT-PCR. Resulting cDNA was then analyzed using Realtime PCR with specific probes to m6A labelled and un-labelled sequences.