Dot blot of Lambda DNA without or with m6A, using MeRIP was performed using M6A and control RNA
Product Details
Product Name Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody [RM362]
Product Description Rabbit monoclonal to N6-Methyladenosine (m6A)
Catalog No. 31-1248-00
Clone Name RM362
Product Data Sheet Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody Data Sheet RM362
Specificity RM362 reacts to N6-Methyladenosine (m6A) in both RNA and DNA. No cross reactivity with non-methylated adenosine.
Immunogen BSA-conjugated N6-Methyladenosine
Application Dot Blot
ELISA
Methylated RNA Immunoprecipitation
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 50 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage DotBlot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.1 ug/mL – 3 ug/mL;
MeRIP: 0.2 ug/mL – 5 ug/mL
Keywords
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Product Images
anti-m6A antibody (RM362). The membrane was
pre-spotted with 5, and 0.5 ng/dot of lambda DNA.
m6A-free DNA was isolated from bacteriophage
lambda grown in an E. coli host-deficient in adenine
methylase (dam-).
from NEB (E1610). Antibodies (Anti-m6A RM362
and Anti-PEG RM105) were mixed for a short time,
with Protein A beads before the addition of m6A
labelled and non- labelled RNA (in equal amounts).
Captured RNA was then washed, eluted and run
through RT-PCR. Resulting cDNA was then analyzed
using Realtime PCR with specific probes to m6A
labelled and un-labelled sequences.
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