Dot blot of DNA using anti-5-caC Rabbit Monoclonal Antibody, Clone RM462. ELISA of DNA using anti-5-caC Rabbit Monoclonal Antibody, Clone RM462 (1 µg/mL).
Product Details
Product Name Anti-5-Carboxylcytosine (5-caC) Rabbit Monoclonal Antibody, Clone RM462
Product Description Rabbit monoclonal to 5-Carboxylcytosine (5-caC) Rabbit Monoclonal Antibody, 5'-caC
Catalog No. 31-1354-00
Clone Name RM462
Product Data Sheet Anti-5-Carboxylcytosine (5-caC) Rabbit Monoclonal Antibody Data Sheet RM462
Specificity RM462 reacts to 5-Carboxylcytosine in DNA. No cross reactivity with non-modified cytosine and other modified cytosines in DNA.
Immunogen KLH-conjugated 5-Carboxylcytosine.
Application Dot Blot
ELISA
Species Reactivity All
Conjugate None
Intended Use Research Use Only
Related Product 5-Carboxylcytosine (5-caC) DNA Matched Antibody Pair
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Dot Blot: 0.1 µg/mL - 1 µg/mL
ELISA: 0.2 µg/mL - 2 µg/mL
Keywords
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Product Images
The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of linear dsDNA (426 bp)
containing either normal cytosines (unmodified), 5-carboxylcytosines (5-caC),
5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or 5-formylcytosines (5-fC).
The plate was coated with different concentrations of linear ssDNA (426 bp) containing
either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines
(5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).
Related Products
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5-Formylcytosines (5-fC) DNA Matched Antibody Pair [RM477/RM476]
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5-Methylcytosine (5-mC) DNA Matched Antibody Pair [RM231/RM476]
DNA Methylation
Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody, Clone RM236; 5′-hmC