Anti-Histone H3 K36M Rabbit Monoclonal Antibody, Clone RM193; Histone H3, K36M Mutant (50ug)

$349.00

Rabbit monoclonal to Histone H3 K36M; Histone H3, K36M Mutant

Product Details
Product NameAnti-Histone H3 K36M Rabbit Monoclonal Antibody [RM193]
Product DescriptionRabbit monoclonal to Histone H3 K36M; Histone H3, K36M Mutant
Catalog No.31-1085-00
Clone NameRM193
Product Data SheetAnti-Histone H3 K36M Rabbit Monoclonal Antibody Data Sheet RM193
SpecificityRM193 reacts to the Histone H3 K36M mutant. No cross reactivity with wild type Histone H3.
ImmunogenA peptide corresponding to Histone H3 K36M mutant
ApplicationWestern Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Chromatin IP
Species ReactivityAll
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size50 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5 ug/mL - 2 ug/mL;
Immunohistochemistry (IHC): 0.5 – 5 ug/mL;
Immunocytochemistry (ICC/IF): 0.5 – 5 ug/mL.
KeywordsH3F3A ; H3F3B ; K36M; bone tumour; chondroblastoma
References for Histone H3 K36M Mutant antibody [RM193]Amary MF et al. The H3F3 K36M mutant antibody is a sensitive and specific marker for the diagnosis of chondroblastoma. Histopathology 10.1111/his.12945 (2016). IHC-P; Human. Read more (PubMed: 26844533)

D. Fang et al. The histone H3.3K36M mutation reprograms the epigenome of chondroblastomas Science 10.1126/science.aae0065 (2016). ChIP; Human. Read more (PubMed: 27229140)
.
Product Images
Western

Western Blot analysis of cell lysates prepared from
293T transfected with a DNA
construct encoding
wild type or K36M mutant proteins of Histone H3.1
and H3.3, using anti-Histone H3 K36M clone RM193.

Immunohistochemical staining of formalin fixed and
paraffin embedded 293T cells transfected with a DNA
construct encoding Histone H3 K36M, stained with
anti-Histone H3 K36M clone RM193.

Immunohistochemical

Immunohistochemical staining of formalin fixed
paraffin embedded (FFPE) Chondroblastoma tumor
cell tissue sections.


Image courtesy of Adrienne Flanagan, Department
of Histopathology, RONH, Stanmore, Middlesex, UK


Related Products

Size Format

25ug – $149, 100ug – $349

Related Products