Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody, Clone RM116

$245.00

Rabbit monoclonal to Human gamma heavy chain

SKU: 31-1018-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody [RM116]
Product DescriptionRabbit Monoclonal to Human Gamma Heavy Chain
Catalog No.31-1018-00
Clone NameRM116
Product Data SheetAnti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody Data Sheet RM116
SpecificityRM116 reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with human IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
ImmunogenHuman IgG
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
Immunohistochemistry

Immunohistochemistry of Human Tonsil using
Anti-Gamma Heavy Chain antibody RM116.

ELISA

ELISA of human immunoglobulins shows RM116
reacted to the γ1, γ2, γ3, γ4 heavy chain of human
IgGs, and the Fc of human IgG. No cross reactivity with
other human heavy chains, mouse IgG, rat IgG,
or goat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL or 50 ng/mL
of RM116 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as the
secondary antibody.

A

A titer ELISA using RM116. The plate was coated
with different amounts of human IgG1. A serial dilution
of RM116 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.

Sandwich ELISA using RM116 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1ug/mL),
followed by an alkaline phosphatase conjugated streptavidin.

Sandwich ELISA using RM116 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1ug/mL),
followed by an alkaline phosphatase conjugated streptavidin.

Cross-activity data of RevMAb anti-human IgG antibodies against
monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL,
50uL/well) of Rhesus monkey IgG (protein A purified from serum).
A serial dilution of anti-human IgG (RM116), anti-human IgG1
(RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119),
(anti-human IgG4 (RM120), anti-human kappa (RM126), and
anti-human lambda (RM127) was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG as the secondary
antibody.


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