Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117

$285.00

Rabbit monoclonal to Human IgG1

SKU: 31-1019-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human IgG1 Rabbit Monoclonal Antibody [RM117]
Product DescriptionRabbit monoclonal to Human IgG1
Catalog No.31-1019-00
Clone NameRM117
Product Data SheetAnti-Human IgG1 Rabbit Monoclonal Antibody Data Sheet RM117
SpecificityRM117 reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
ImmunogenPeptide corresponding to the hinge region of Human IgG1
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
Sandwich

Sandwich ELISA using RM117 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.

Sandwich

Sandwich ELISA using RM117 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.

ELISA

ELISA of human immunoglobulins shows RM117
only reacted to human IgG1. No cross reactivity
with Human IgG2, IgG3, IgG4, IgE, IgD, IgA,
mouse IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

A

A titer ELISA using RM117. The plate was
coated with different amounts of human IgG1.
A serial dilution of RM117 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.

Cross-activity data of RevMAb anti-human IgG antibodies against
monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL,
50uL/well) of Rhesus monkey IgG (protein A purified from serum).
A serial dilution of anti-human IgG (RM116), anti-human IgG1
(RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119),
(anti-human IgG4 (RM120), anti-human kappa (RM126), and
anti-human lambda (RM127) was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG as the secondary
antibody.


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