Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM161; H3K9ac, Histone H3 (K9 acetylation)

$155.00

Rabbit monoclonal to Acetylated Histone H3 Lysine 9 (K9ac), H3K9ac, Histone H3 (K9 acetylation)

SKU: 31-1054-00.

Product Details
Product NameAnti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM161]
Product DescriptionRabbit monoclonal to Acetylated Histone H3 Lysine 9 (K9ac), H3K9ac, Histone H3 (K9 acetylation)
Catalog No.31-1054-00
Clone NameRM161
Product Data SheetAnti-Acetyl-Histone H3 Lys9 Rabbit Monoclonal Antibody Data Sheet RM161
SpecificityRM161 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9).
ApplicationWestern Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.25 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ChIP: 2 ug/mL-10 ug/mL;
IHC: 0.1 ug/mL – 1 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL.

Keywords
References for Acetyl-Histone H3 (Lys9) antibody [RM161]Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)

Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153)
Product Images
RM161

RM161 specifically reacts to Histone H3 acetylated
at Lysine 9 (K9ac). No cross reactivity with acetyl-
ated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18
(K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac),
Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79
(K79ac), or Lysine 122 (K122) in histone H3.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM161 at 0.25 ug/mL, showed a band of
histone H3 acetylated at Lysine 9 in treated HeLa.

Sandwich

Sandwich ELISA against acetylated histone H3 at Lys 9
using HeLa whole cell lysate, treated or untreated with
Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as
the capture antibody and biotinylated anti-H3K9ac (RM161,
1 ug/mL) as the detection antibody.

ChIP

ChIP performed on HeLa cells using H3K9ac antibody (RM161,
5ug). Real-time PCR was performed using primers specific to
the gene indicated.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys9)
Rabbit mAb RM161 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

Immunohistochemistry

Immunohistochemistry staining of HepG2 cells using
anti-Acetyl-Histone H3 (Lys9) antibody, RM161.


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Size Format

25ug – $155, 100ug – $369

References

Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)

Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153)