RM161 specifically reacts to Histone H3 acetylated Western Blot of acid extracts from HeLa cells Sandwich ELISA against acetylated histone H3 at Lys 9 ChIP performed on HeLa cells using H3K9ac antibody (RM161, Immunocytochemistry of HeLa cells treated with Immunohistochemistry staining of HepG2 cells using
Product Details
Product Name Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM161]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 9 (K9ac), H3K9ac, Histone H3 (K9 acetylation)
Catalog No. 31-1054-00
Clone Name RM161
Product Data Sheet Anti-Acetyl-Histone H3 Lys9 Rabbit Monoclonal Antibody Data Sheet RM161
Specificity RM161 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9).
Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.25 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ChIP: 2 ug/mL-10 ug/mL;
IHC: 0.1 ug/mL – 1 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL.
Keywords
References for Acetyl-Histone H3 (Lys9) antibody [RM161] Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)
Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153)
Product Images
at Lysine 9 (K9ac). No cross reactivity with acetyl-
ated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18
(K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac),
Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79
(K79ac), or Lysine 122 (K122) in histone H3.
untreated (-) or treated with sodium butyrate (+),
using RM161 at 0.25 ug/mL, showed a band of
histone H3 acetylated at Lysine 9 in treated HeLa.
using HeLa whole cell lysate, treated or untreated with
Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as
the capture antibody and biotinylated anti-H3K9ac (RM161,
1 ug/mL) as the detection antibody.
5ug). Real-time PCR was performed using primers specific to
the gene indicated.
sodium butyrate, using Acetyl-Histone H3 (Lys9)
Rabbit mAb RM161 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
anti-Acetyl-Histone H3 (Lys9) antibody, RM161.
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| Size Format | 25ug – $188, 100ug – $419 |
|---|
References
Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)
Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153)