Anti-Human IgG2 Rabbit Monoclonal Antibody, Clone RM118

$299.00

Rabbit monoclonal to Human IgG2

SKU: 31-1020-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human IgG2 Rabbit Monoclonal Antibody [RM118]
Product DescriptionRabbit monoclonal to Human IgG2
Catalog No.31-1020-00
Clone NameRM118
Product Data SheetAnti-Human IgG2 Rabbit Monoclonal Antibody Data Sheet RM118
SpecificityRM118 reacts to the heavy chain of human IgG2. RM118 does not cross react to any other IgG subclasses (IgG1, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, or IgE. RM118 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
ImmunogenHuman IgG2
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL.
Keywords
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Product Images
Detection

Detection of human IgG2 in monkey serum.
Sandwich ELISA using RM118 as the capture
antibody, and biotinylated anti-human light
chains ( κ+ λ) antibody RM129 as the detection
antibody, followed by a HRP conjugated streptavidin.

Sandwich

Sandwich ELISA using RM118 as the capture
antibody, and biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.

ELISA

ELISA showing RM118 reacts only to human IgG2,
and not to any other IgG subclasses (IgG1, IgG3,
or IgG4), and no cross reactivity to IgM, IgA, IgD,
IgE, mouse IgG, rat IgG, or goat IgG.

A

A titer ELISA using RM118. The plate was
coated with different amounts of human IgG2.
A serial dilution of RM118 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.

ELISA

ELISA showing RM118 does not react to monkey
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM118 and a monkey IgG binding
antibody (positive control) was used as the detection
antibody.


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