Western Blot of (1) acid extracts of HeLa cells treated with RM179 specifically reacts to Histone H3 acetylated at Lysine 56 Immunocytochemistry of HeLa cells treated with Immunohistochemistry staining of HepG2 cells using
Product Name Anti-Acetyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody [RM179]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 56 (K56ac), H3K56ac, Histone H3 (K56 acetylation)
Catalog No. 31-1078-00
Clone Name RM179
Product Data Sheet Anti-Acetyl-Histone H3 Lys56 Rabbit Monoclonal Antibody Data Sheet RM179
Specificity RM179 reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys56).
Application Western Blot (WB)
Chromatin IP (ChIP)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
IHC: 1 ug/mL – 10 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL.
Keywords References for Acetyl-Histone H3 (Lys56) antibody [RM179] Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)
sodium butyrate and (2) recombinant histone H3.3, using
RM179 at 1 µg/mL, showed a band of histone H3 acetylated
at Lysine 56 in treated HeLa.
(K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl),
acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),
Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36
(K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
sodium butyrate, using Acetyl-Histone H3 (Lys56)
Rabbit mAb RM179 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
Anti-Acetyl-Histone H3 (Lys56) antibody, RM179.
Western Blot of (1) acid extracts of HeLa cells treated with
RM179 specifically reacts to Histone H3 acetylated at Lysine 56
Immunocytochemistry of HeLa cells treated with
Immunohistochemistry staining of HepG2 cells using