Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone RM127

$265.00

Rabbit monoclonal to Human lambda light chain

SKU: 31-1029-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human Lambda Light Chain Rabbit Monoclonal Antibody [RM127]
Description Rabbit monoclonal to Human Lambda Light Chain
Catalog No.31-1029-00
Clone NameRM127
Product Data SheetAnti-Human Lambda Light Chain Rabbit Monoclonal Antibody Data Sheet RM127
SpecificityRM127 reacts to the lambda light chain of human immunoglobulins, and does not react to the kappa light chain. RM127 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
ImmunogenHuman IgG
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100 µg
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.05ug/mL – 0.2ug/mL;
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
References for Human Ig Lambda Light Chain antibody [RM127]Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)
Product Images
Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded human tonsil tissue section using
anti-human Ig lambda light chain rabbit monoclonal
antibody (Clone RM127).

ELISA

ELISA showing RM127 reacts only to the lambda
light chain of human immunoglobulins, and not to
the kappa light chain, mouse IgG, rat IgG, or goat IgG.

A

A titer ELISA using RM127. The plate was coated
with different amounts of human IgG3λ. A serial
dilution of RM127 was used as the primary antibody,
followed by an alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.

ELISA

ELISA showing RM127 does not react to monkey
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM127 and a monkey IgG binding
antibody (positive control) was used as the detection
antibody.


Related Products

References

Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)