Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag

$310.00

Biotinylated Rabbit monoclonal to His-Tag (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)

Product Details
Product NameBiotin Anti-His-Tag (6xHis-Tag or 10xHis-Tag) Rabbit Monoclonal Antibody [RM146]
Product DescriptionBiotinylated Rabbit monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
Catalog No.31-1048-02
Clone NameRM146
Product Data SheetBiotin Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146
SpecificityRM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
ImmunogenMixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
ApplicationWestern Blot
ELISA
Immunoprecipitation
Immunocytochemistry
Flow Cytometry
Immunohistochemistry
Species ReactivityAll
ConjugateBiotin
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size50 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.1 ug/mL – 0.5 ug/mL;
Immunocytochemistry 0.5 ug/mL – 2 ug/mL;
Flow Cytometry: 0.5 ug/mL – 2 ug/mL;
Immunohistochemistry; 0.1 ug/mL-1 ug/mL;
ELISA: 0.01 ug/mL-0.5 ug/mL.
Keywords
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Product Images
A

A titer ELISA performed on purified HisTag-PCSK9
recombinant protein. A serial dilution of Biotin Anti-
HisTag Rabbit Monoclonal Antibody Clone RM146
was used as the primary antibody, followed by
alkaline phosphatase conjugated Streptavidin.

Western

Western blot of 293T cells transfected (+) or untrans-
fected (-) with a DNA construct encoding His-Tag
Histone H3 (G34W) protein, using Biotin Anti-HisTag
Rabbit Monoclonal Antibody Clone RM146 at 0.2 ug/mL,
followed by a HRP conjugated Streptavidin.

Immunohistochemistry

Immunohistochemistry staining of naïve HepG2 cells
(Negative control) using anti-His-Tag antibody, RM146.

Immunohistochemistry

Immunohistochemistry staining of 293T cells expressing
His-Tag nuclear protein X, using anti-His-Tag antibody,
RM146.


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