Sandwich ELISA performed on whole lysate of LNCaP Western blot of 293T cells transfected (+) or non-
Product Details
Product Name Anti-HA-Tag Rabbit/Human Chimeric Monoclonal Antibody, [RMH02]
Product Description Rabbit/Human Chimeric monoclonal to HA-Tag
This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HA-Tag monoclonal antibody (RM305), with the constant domain of Human IgG1. This antibody is recognized only by an anti-Human IgG secondary antibody.
Catalog No. 54-1191-00
Clone Name RMH02
Product Data Sheet Anti-HA-Tag Rabbit/Human Chimeric Monoclonal Antibody Data Sheet RMH02
Specificity RMH02 reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.
Immunogen A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106).
Application Western Blot
ELISA (Capturer or Detector)
Species Reactivity All
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit/Human Chimeric
Rabbit Fv/ Human IgG1 CH and Cκ
Usage ELISA: Capturer: 0. 5 ug/mL – 2 ug/mL;
Detector: 0.01 ug/mL – 0.5 ug/mL;
Western Blot: 0.1 ug/mL – 1 ug/mL.
Keywords
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Product Images
cells overexpressing HA-tag Bag1 protein. ELISA plate
was coated with 50uL/well of chimeric human anti-HA-
Tag antibody Clone RMH02 (1ug/mL) as the capture
antibody. Anti-Bag1 rabbit serum was used as the
detection antibody, followed by an alkaline phosphatase
conjugated anti-Rabbit IgG as the Secondary antibody.
transfected (-) with a DNA construct encoding HA-
Tag KRas fusion protein, using anti-HA-tag rabbit
monoclonal antibody (clone RM305) at 0.1 ug/mL,
followed by a HRP conjugated anti-Human IgG
secondary antibody.
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