Western blot of 293 cell or E.coli whole cell lysate with or without N-terminal His ELISA comparison of a N-terminal His tagged fusion protein (50ug/well) and a Western blots showing non-specific reactivities of anti-HisTag rabbit monoclonal Western blots showing non-specific reactivities of anti-HisTag rabbit monoclonal Immunostaining of FFPE 293 cells overexpressing a
Product Details
Product Name Anti-His-Tag Rabbit Monoclonal Antibody [RM146X]
Product Description Rabbit monoclonal to His-Tag at either the N-Terminus (amino) or C-Terminus (carboxy)
Catalog No. 38-1048-00
Clone Name RM146X
Product Data Sheet Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146X
Specificity RM146X reacts to recombinant proteins containing the His-Tag fused to either the amino or carboxy terminus. Very low non-specific reactivity with endogenous proteins in mammalian or bacteria cells, compared with other anti-HisTag antibodies.
Immunogen 6xHis-Tag coupled to KLH
Application Western Blot
ELISA
Immunoprecipitation
Immunocytochemistry
Species Reactivity All
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 0.01 ug/mL – 0.1 ug/mL;
ELISA: 0.01 ug/mL – 0.2 ug/mL;
IP: 0.5 ug/mL – 2 ug/mL;
FFPE ICC: 0.5 ug/mL – 2 ug/mL.
IHC: 0.004 ug/mL - 0.1 ug/mL
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Product Images
tagged fusion protein X or C-terminal His tagged fusion protein Y, using
anti-HisTag rabbit monoclonal antibody, RM146X (0.02 ug/mL).
C-terminal His tagged fusion protein (50ug/well), using our anti-HisTag rabbit
monoclonal antibody, RM146X, versus a leading anti-HisTag mouse monoclonal
antibody.
antibody RM146X with endogenous proteins in mammalian 293 cells bacteria cells, compared with a leading anti-HisTag mouse monoclonal antibody.
antibody RM146X with endogenous proteins in mammalian E.coli bacteria cells,
compared with a leading anti-HisTag mouse monoclonal antibody.
cytoplasmic or nuclear protein, using anti-His-tag rabbit
monoclonal antibody (clone RM146X) at 4 ng/mL
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