Sandwich ELISA performed on different amounts of A titer ELISA performed on purified HisTag-PCSK9 Western blot of 293T cells transfected (+) or untransfected (-)
Product Details
Product Name Anti-His-Tag Chimeric Human Monoclonal Antibody, [RMH01]
Product Description Chimeric Human monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
Catalog No. 54-1161-00
Clone Name RMH01
Product Data Sheet Anti-His-Tag Chimeric Human Monoclonal Antibody Data Sheet RMH01
Specificity RMH01 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein in mammalian or bacteria cells.
Immunogen Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Application Western Blot
ELISA (Capturer or Detector)
Species Reactivity All
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: Capturer: 0. 1 ug/mL – 2 ug/mL;
Detector: 0.01 ug/mL – 0.5 ug/mL;
Western Blot: 0.1 ug/mL – 1 ug/mL.
Keywords
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Product Images
whole lysate of 293T cells transfected with a DNA
construct encoding HisTag Histone H3 (K36M) protein,
using 50uL/well of different concentrations of chimeric
human anti-HisTag antibody Clone RMH01 as the
capture antibody. Anti-H3K36M rabbit monoclonal
antibody (RM193) was used as the detection antibody,
followed by an alkaline phosphatase conjugated Rabbit
IgG as the Secondary antibody.
recombinant protein. A serial dilution of chimeric
human anti-HisTag antibody Clone RMH01 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-Human IgG as the secondary antibody.
with a DNA construct encoding His-Tag Histone H3 (G34W)
protein, using chimeric human anti-HisTag antibody Clone RMH01
at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG
secondary antibody.
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