Western Blot of recombinant Histone H2A, H2B, Western Blot of A375, HEK293, HeLa and SK-MEL-2 Immunocytochemistry of HeLa cells, using Anti-
Product Name Anti-Histone H4 Rabbit Monoclonal Antibody [RM212]
Product Description Rabbit monoclonal to Histone H4
Catalog No. 31-1089-00
Clone Name RM212
Product Data Sheet Anti-Histone H4 Rabbit Monoclonal Antibody Data Sheet RM212
Specificity RM212 reacts to the Histone H4 protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Immunogen A peptide corresponding to the C-terminus of human Histone H4.
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.1 ug/mL – 0.5 ug/mL;
ICC: 1 μg/mL - 2 μg/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords . Product Images
H3.1, H3.3 and H4 proteins, using RM212 at
0.2 ug/mL. RM212 is specific to Histone H4.
whole cell lysates, using RM212 at 0.2 ug/mL, showed
endogenous Histone H4 in A375, HEK293, HeLa and
Histone H4 Rabbit mAb RM212 (red). Actin filaments
have been labeled with fluorescein phalloidin (green).
Western Blot of recombinant Histone H2A, H2B,
Western Blot of A375, HEK293, HeLa and SK-MEL-2
Immunocytochemistry of HeLa cells, using Anti-