Western Blot of recombinant Histone H2A, H2B, Western Blot of A375, HEK293, HeLa and SK-MEL-2 Immunocytochemistry of HeLa cells, using Anti-
Product Details
Product Name Anti-Histone H4 Rabbit Monoclonal Antibody [RM212]
Product Description Rabbit monoclonal to Histone H4
Catalog No. 31-1089-00
Clone Name RM212
Product Data Sheet Anti-Histone H4 Rabbit Monoclonal Antibody Data Sheet RM212
Specificity RM212 reacts to the Histone H4 protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Immunogen A peptide corresponding to the C-terminus of human Histone H4.
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.1 ug/mL – 0.5 ug/mL;
ICC: 1 μg/mL - 2 μg/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
H3.1, H3.3 and H4 proteins, using RM212 at
0.2 ug/mL. RM212 is specific to Histone H4.
whole cell lysates, using RM212 at 0.2 ug/mL, showed
endogenous Histone H4 in A375, HEK293, HeLa and
SK-MEL-2 cells.
Histone H4 Rabbit mAb RM212 (red). Actin filaments
have been labeled with fluorescein phalloidin (green).
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