Anti-cAMP Rabbit Monoclonal Antibody, Clone RM466
$377.00
Rabbit monoclonal to cAMP (Cyclic Adenosine Monophosphate)
Related Product: Catalog# 71-1358-00, cAMP-HRP
Competitive ELISA of cAMP using anti-cAMP (RM466). The 96-well plate was coated Competitive ELISA showing the specificity of anti-cAMP (RM466). Competitive ELISA detecting cAMP in HeLa cells. The 96-well plate was
Product Details
Product Name Anti-cAMP Rabbit Monoclonal Antibody [RM466]
Product Description Rabbit monoclonal to cAMP (Cyclic Adenosine Monophosphate)
Catalog No. 31-1358-00
Clone Name RM466
Product Data Sheet Anti-cAMP Rabbit Monoclonal Antibody Data Sheet RM466
Specificity RM466 reacts to cAMP (Cyclic Adenosine Monophosphate). No cross reactivity with other cyclic nucleotide or nucleoside phosphate.
Immunogen KLH-conjugated cAMP
Application ELISA
Conjugate None
Intended Use Research Use Only
Related Product Antigen for Competitive Assay 71-1358-00 - cAMP-HRP
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50ug
Concentration 1 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.05 ug/mL – 0.5 ug/mL
Keywords
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Product Images
with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-cAMP(RM466) (50 µl/well)
was added and incubated. After wash and block, different concentrations of cAMP (25 µl/well)
were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cAMP. (71-1358-00)
TMB was used to develop the color after incubation and wash.
The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well).
0.05 µg/ml of anti-cAMP (RM466) (50 µl/well) was added and incubated.
After wash and block, cAMP and other cyclic nucleotides or nucleoside
phosphates (25 µl/well) were added along with 25 µl/well of 1/5,000
diluted HRP conjugated cAMP (71-1358-00). TMB was used to develop the
color after incubation and wash.
coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml
of anti-cAMP (RM466) (50 µl/well) was added and incubated. After wash and
block, HeLa cell lysate (~105cell/mL) or control (lysate buffer only) (25 µl/well)
were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cAMP (71-1358-00).
TMB was used to develop the color after incubation and wash.
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