Competitive ELISA of cAMP using anti-cAMP (RM466). The 96-well plate was coated Competitive ELISA showing the specificity of anti-cAMP (RM466).
Product Details
Product Name cAMP-HRP (Peroxidase-Conjugated Cyclic Adenosine Monophosphate) [RM-BA466]
Catalog No. 71-1358-00
Product Description RevMAb cAMP-peroxidase conjugate is 8-[HRP]-AET-cAMP.
It is suitable for RevMAb anti-cAMP rabbit monoclonal antibody (clone RM466) in immunoassay development for cAMP detection.
Product Data Sheet cAMP-HRP Datasheet
Application ELISA
Intended Use Research Use Only
Related Products cAMP-HRP is antigen for Competitive Assay with Anti-cAMP Rabbit Monoclonal Antibody [RM466]
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Properties
Form Liquid
Volume/Item Size 100 uL
Storage Buffer 50% Glycerol/PBS with 1% BSA
Stability Stable for 1 Year at -20.0°C from date of receipt
Usage ELISA: 1:2,000 – 1:10,000
(The appropriate working concentrations may be affected by antibody affinity, the method of detection, temperature, the reaction time, and other factors.)
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Product Images
with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-cAMP(RM466) (50 µl/well)
was added and incubated. After wash and block, different concentrations of cAMP (25 µl/well)
were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cAMP, 71-1358-00
TMB was used to develop the color after incubation and wash.
The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well).
0.05 µg/ml of anti-cAMP (RM466) (50 µl/well) was added and incubated.
After wash and block, cAMP and other cyclic nucleotides or nucleoside
phosphates (25 µl/well) were added along with 25 µl/well of 1/5,000
diluted HRP conjugated cAMP, 71-1358-00. TMB was used to develop the
color after incubation and wash.
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Matched Antibody Pair Kits
Biotin Conjugates
Biotinylated Anti-25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes, Clone RM428
Primary Antibodies
Anti-25-OH Vitamin D3 Rabbit/Human Chimeric Monoclonal Antibody, Clone RMH04
Primary Antibodies
Anti-25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes, Clone RM428
Related Products
Small Molecule
Small Molecule
Small Molecule
Matched Antibody Pair Kits
Biotin Conjugates
Biotinylated Anti-25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes, Clone RM428
Primary Antibodies
Anti-25-OH Vitamin D3 Rabbit/Human Chimeric Monoclonal Antibody, Clone RMH04
Primary Antibodies
Anti-25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes, Clone RM428