Competitive ELISA of cGMP using anti-cGMP (RM467). The 96-well plate was Competitive ELISA showing the specificity of anti-cGMP (RM467). The 96-well plate was
Product Details
Product Name cGMP-HRP (Peroxidase-Conjugated Cyclic Guanosine Monophosphate)
Catalog No. 71-1359-00
Product Description RevMAb cGMP-peroxidase conjugate is 8-[HRP]-AET-cGMP.
It is suitable for RevMAb anti-cGMP rabbit monoclonal antibofy (clone RM467) in immunoassay development for cGMP detection.
Product Data Sheet cGMP-HRP Datasheet
Application ELISA
Intended Use Research Use Only
Related Products cGMP-HRP antigen for Competitive Assay withAnti-cGMP Rabbit Monoclonal Antibody [RM467]
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Properties
Form Liquid
Volume/Item Size 100 uL
Storage Buffer 50% Glycerol/PBS with 1% BSA
Stability Stable for 1 Year at -20.0°C from date of receipt
Usage ELISA: 1:2,000 – 1:10,000
(The appropriate working concentrations may be affected by antibody affinity, the method of detection, temperature, the reaction time, and other factors.)
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Product Images
coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-
cGMP (RM467) (50 µl/well) was added and incubated. After wash and block,
different concentrations of cGMP (25 µl/well) were added along with 25 µl/well of
1/5,000 diluted HRP conjugated cGMP, 71-1359-00. TMB was used to develop
the color after incubation and wash.
coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-cGMP (RM467)
(50 µl/well) was added and incubated. After wash and block, cGMP and other cyclic
nucleotides or nucleoside phosphates (25 µl/well) were added along with 25 µl/well
of 1/5,000 diluted HRP conjugated cGMP, 71-1359-00. TMB was used to
develop the color after incubation and wash.
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Matched Antibody Pair Kits
Biotin Conjugates
Biotinylated Anti-25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes, Clone RM428
Primary Antibodies
Anti-25-OH Vitamin D3 Rabbit/Human Chimeric Monoclonal Antibody, Clone RMH04
Primary Antibodies
Anti-25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes, Clone RM428
Related Products
Small Molecule
Small Molecule
Small Molecule
Matched Antibody Pair Kits
Biotin Conjugates
Biotinylated Anti-25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes, Clone RM428
Primary Antibodies
Anti-25-OH Vitamin D3 Rabbit/Human Chimeric Monoclonal Antibody, Clone RMH04
Primary Antibodies
Anti-25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 Complexes, Clone RM428