Sandwich ELISA using RM122 as the capture antibody ELISA of human immunoglobulins shows RM122 reacts A titer ELISA using RM122. The plate was coated
Product Details
Product Name Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122
Product Description Rabbit monoclonal to Human IgE
Catalog No. 31-1024-00
Clone Name RM122
Product Data Sheet Anti-Human IgE Rabbit Monoclonal Antibody Data Sheet RM122
Specificity RM122 reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.
Immunogen Human IgE
Application ELISA
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 10 ng/well – 100 ng/well (for Capture);
0.01 µg/mL – 0.1 µg/mL (for Detection);
Keywords
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Product Images
(25 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
only to human IgEλ from human myeloma plasma
and the IgE from human plasma. No cross reactivity
with Human IgG, IgM, IgD, or IgA. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL or 10 ng/mL of RM122 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
with different amounts of human IgE. A serial dilution
of RM122 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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