Sandwich ELISA using RM121 as the capture antibody Sandwich ELISA using RM121 as the capture antibody ELISA of human immunoglobulins shows RM121 A titer ELISA using RM121. The plate was coated
Product Details
Product Name Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121
Product Description Biotinylated Rabbit monoclonal to Human IgM
Catalog No. 31-1023-02
Clone Name RM121
Product Data Sheet Biotin Anti-Human IgM Rabbit Monoclonal Antibody Data Sheet RM121
Specificity RM121 reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
Immunogen Human IgM
Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL.
Keywords
.
Product Images
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
reacts only to human IgM. No cross reactivity
with Human IgG, IgE, IgD, or IgA. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL of RM121 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.
with different amounts of human IgM. A serial dilution
of RM121 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
Related Products
Biotin Conjugated Human Ig
Biotin Anti-Human IgG4 Fc Rabbit Monoclonal Antibody, Clone RM217
Biotin Conjugated Human Ig
Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody, Clone RM118
Biotin Conjugated Human Ig
Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120
Biotin Conjugated Human Ig
Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117
Biotin Conjugated Human Ig
Biotin Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122
Biotin Conjugated Human Ig
Biotin Anti-Human IgA (α1 & α2) Rabbit Monoclonal Antibody, Clone RM128
Biotin Conjugated Human Ig
Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody, Clone RM125
Biotin Conjugated Human Ig
Biotin Anti-Human IgA1 Rabbit Monoclonal Antibody, Clone RM124
Biotin Conjugated Human Ig
Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121
Biotin Conjugated Human Ig
Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119
Biotin Conjugated Human Ig
Biotin Anti-Ig Human Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM126
Biotin Conjugated Human Ig
Biotin Anti-Human IgG Rabbit Monoclonal Antibody, Clone RM116