Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM181; H3K79me2, Histone H3 (dimethyl K79)

$155.00$360.00

Rabbit monoclonal to Dimethylated Histone H3 Lysine 79, H3K79me2, Histone H3 (dimethyl K79)

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Product Details
Product NameAnti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [RM181]
Product DescriptionRabbit monoclonal to Dimethylated Histone H3 Lysine 79, H3K79me2, Histone H3 (dimethyl K79)
Catalog No.31-1065-00
Clone NameRM181
Product Data SheetAnti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM181
SpecificityRM181 reacts to Histone H3 dimethylated at Lysine 79 (K79me2). ). Very slightly cross-reacts with monomethylated Lysine 79 (K79me1) at high concentration. No cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.
ImmunogenA dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79).
ApplicationWestern Blot (WB)
Chromatin IP (ChIP)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.25 ug/mL – 1 ug/mL;
IHC: 0.1 ug/mL – 1 ug/mL;
ChIP: 2 ug/mL- 10 ug.mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
RM181

RM181 specifically reacts to Histone H3 dimethylated
at Lysine 79 (K79me2). Very slightly cross reactivity
with monomethylated Lysine 14 (K14me1), and no
cross reactivity with non-modified Lysine 79 (K79 ctrl),
trimethylated Lysine 79 (K79me3), or other methylations
in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
and acid extracts of HeLa cells (2), using RM181
at 0.25 ug/mL, showed a band of histone H3
dimethylated at Lysine 79 (K79me2) in HeLa cells.

ChIP

ChIP performed on HeLa cells using H3K79me2 antibody
(RM181, 5ug). Real-time PCR was performed using primers
specific to the gene indicated.

Immunohistochemistry

Immunohistochemistry staining of HepG2 cells using
Anti-Dimethyl-Histone H3 (Lys79) antibody, RM181.


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