RM175 specifically reacts to Histone H3 Western Blot of recombinant histone H3.3 (1) and Immunohistochemistry staining of HepG2 cells using
Product Name Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [RM175]
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27)
Catalog No. 31-1062-00
Clone Name RM175
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody Data Sheet RM175
Specificity RM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).
Application Chromatin IP (ChIP)
Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ChIP: 1µg-5 µg;
Western Blot: 1 ug/mL – 2 ug/mL;
IHC: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
References for Trimethyl-Histone H3 (Lys27) antibody [RM175] Tsai CC et al. Overexpression of Histone H3 Lysine 27 Trimethylation Is Associated With Aggressiveness and Dedifferentiation of Thyroid Cancer. Endocr Pathol. (2019), 10.1007/s12022-019-09586-1. IHC; Human. Read more (PubMed: 31396854)
Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)
. Product Images
trimethylated at Lysine 27 (K27me3). No
cross reactivity with non-modified Lysine 27
(K27 Ctrl), monomethylated Lysine 27
(K27me1) or dimethylated Lysine 27 (K27me2),
or other methylations in Histone H3.
acid extracts of HeLa cells (2), using RM175 at
1 ug/mL, showed a band of histone H3 trimethyl-
ated at Lysine 27 (K27me3) in HeLa cells.
Anti-Trimethyl-Histone H3 (Lys27) antibody, RM175.
RM175 specifically reacts to Histone H3
Western Blot of recombinant histone H3.3 (1) and
Immunohistochemistry staining of HepG2 cells using