RM175 specifically reacts to Histone H3 Western Blot of recombinant histone H3.3 (1) and Immunohistochemistry staining of HepG2 cells using
Product Details
Product Name Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [RM175]
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27)
Catalog No. 31-1062-00
Clone Name RM175
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody Data Sheet RM175
Specificity RM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).
Application Chromatin IP (ChIP)
Western Blot (WB)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ChIP: 1µg-5 µg;
Western Blot: 1 ug/mL – 2 ug/mL;
IHC: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
References for Trimethyl-Histone H3 (Lys27) antibody [RM175] Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)
.
Product Images
Related Products
Histone H3 Acetylation
Histone H3 Methylation
Histone H3 Methylation
Histone H3 Methylation
Histone H3 Methylation
Histone H3 Methylation
Histone H3 Methylation
Primary Antibodies
Histone H3 Methylation
Primary Antibodies
Histone H3 Methylation
Histone H3 Methylation
| Size Format | 25ug – $165, 100ug – $399 |
|---|
References
Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)
Related products
Primary Antibodies
Primary Antibodies
Anti-Human IgA (α1 & α2) Rabbit Monoclonal Antibody, Clone RM128
Primary Antibodies
Primary Antibodies
Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129
Primary Antibodies
Primary Antibodies
Anti-Human Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM126
Primary Antibodies
Primary Antibodies
Anti-Acetylated-Lysine Rabbit Monoclonal Antibody, Clone RM101
Primary Antibodies
Anti-Phosphotyrosine Rabbit Monoclonal Antibody, Clone RM111
Primary Antibodies
IVD Raw Materials