Anti-Dimethyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM165; H3K14me2, Histone H3 (dimethyl K14)

$155.00

Rabbit monoclonal to Dimethylated Histone H3 Lysine 14, H3K14me2, Histone H3 (dimethyl K14)

SKU: 31-1060-00.

Product Details
Product NameAnti-Dimethyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [RM165]
Product DescriptionRabbit monoclonal to Dimethylated Histone H3 Lysine 14, H3K14me2, Histone H3 (dimethyl K14)
Catalog No.31-1060-00
Clone NameRM165
Product Data SheetAnti-Dimethyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody Data Sheet RM165
SpecificityRM165 reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in histone H3.
ImmunogenA dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys14).
ApplicationWestern Blot (WB)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.25 ug/mL – 1 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
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Product Images
RM165

RM165 specifically reacts to Histone H3 dimethylated
at Lysine 14 (K14me2). No cross reactivity with non
-modified Lysine 14 (H3K14), monomethylated
Lysine14 (K14me1), trimethylated Lysine 14
(K14me3), or other methylations in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
and acid extracts of HeLa cells (2), using RM165
at 0.25 ug/mL, showed a band of histone H3
dimethylated at Lysine 14 (K14me2) in HeLa cells.


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