Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151; H3K9me2, Histone H3 (dimethyl K9)

$155.00$365.00

Rabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2, Histone H3 (dimethyl K9)

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Product Details
Product NameAnti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM151]
Product DescriptionRabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2, Histone H3 (dimethyl K9)
Catalog No.31-1059-00
Clone NameRM151
Product Data SheetAnti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM151
SpecificityRM151 reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in histone H3.
ImmunogenA dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9).
ApplicationWestern Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt.
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.25 ug/mL – 1 ug/mL;
ChIP: 2 ug/mL- 10 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
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Product Images
RM151

RM151 specifically reacts to Histone H3 dimethylated
at Lysine 9 (K9me2). No cross reactivity with non
-modified Lysine 9 (H3 1-19), monomethylated
Lysine 9 (K9me1), trimethylated Lysine 9
(K9me3), or other methylations in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
and acid extracts of HeLa cells (2), using RM151
at 0.5 ug/mL, showed a band of histone H3
dimethylated at Lysine 9 (K9me2) in HeLa cells.

ChIP

ChIP performed on HeLa cells using H3K9me2
antibody (RM151, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Dimethyl-Histone H3
(Lys9) Rabbit mAb RM151 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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25ug – $155, 100ug – $365

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